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Laser capture: what is it?

This is a brief Wikipedia article that has some basic information about laser microdissection.

Laser Capture Microdissection (LCM ): Beam Me Up. 

Laser-Capture Microdissection Advances.


Selected references for laser microdissection.


Laser capture microdissection.
Emmert-Buck MR, Bonner RF, Smith PD, Chuaqui RF, Zhuang Z, Goldstein SR, Weiss RA, Liotta LA.

The classic paper that started it all!

A quantitative proteomic workflow for characterization of frozen clinical biopsies: Laser capture microdissection coupled with label-free mass spectrometry.                                                                           Shapiro JP, Biswas S, Merchant AS, Satoskar A, Taslim C, Lin S, Rovin BH, Sen CK, Roy S, Freitas MA.

 Immuno-LCM: laser capture microdissection of immunostained frozen sections for mRNA analysis.
Fend F, Emmert-Buck MR, Chuaqui R, Cole K, Lee J, Liotta LA, Raffeld M.

 Use of laser-capture microdissection for the identification of marker genes for the ventromedial hypothalamic nucleus.
Segal JP, Stallings NR, Lee CE, Zhao L, Socci N, Viale A, Harris TM, Soares MB, Childs G, Elmquist JK, Parker KL, Friedman JM.

 Histological staining methods preparatory to laser capture microdissection significantly affect the integrity of the cellular RNA.
Wang H, Owens JD, Shih JH, Li MC, Bonner RF, Mushinski JF.

 Laser-capture microdissection.
Espina V, Wulfkuhle JD, Calvert VS, VanMeter A, Zhou W, Coukos G, Geho DH, Petricoin EF 3rd, Liotta LA.

 Expression profiling of intermingled long-range projection neurons harvested by laser capture microdissection.
Lombardino AJ, Hertel M, Li XC, Haripal B, Martin-Harris L, Pariser E, Nottebohm F.

 Contaminating cells alter gene signatures in whole organ versus laser capture microdissected tumors: a comparison of experimental breast cancers and their lymph node metastases.
Harrell JC, Dye WW, Harvell DM, Sartorius CA, Horwitz KB.

 Transcriptome-wide analysis of blood vessels laser captured from human skin and chronic wound-edge tissue.
Roy S, Patel D, Khanna S, Gordillo GM, Biswas S, Friedman A, Sen CK.

 Differentiating proteomic biomarkers in breast cancer by laser capture microdissection and MALDI MS.
Sanders ME, Dias EC, Xu BJ, Mobley JA, Billheimer D, Roder H, Grigorieva J, Dowsett M, Arteaga CL, Caprioli RM.

 Laser microdissection and capture of pure cardiomyocytes and fibroblasts from infarcted heart regions: perceived hyperoxia induces p21 in peri-infarct myocytes.
Kuhn DE, Roy S, Radtke J, Khanna S, Sen CK.

 Microdissecting the proteome.
von Eggeling F, Melle C, Ernst G.

 An overview of laser microdissection technologies.
Murray GI.

 Simultaneous isolation of DNA and RNA from the same cell population obtained by laser capture microdissection for genome and transcriptome profiling.
Xu C, Houck JR, Fan W, Wang P, Chen Y, Upton M, Futran ND, Schwartz SM, Zhao LP, Chen C, Mendez E.

 Tissue microdissection.
Erickson HS, Gillespie JW, Emmert-Buck MR.

 High quality RNA from multiple brain regions simultaneously acquired by laser capture microdissection.
Wang WZ, Oeschger FM, Lee S, Molnár Z.

 Optimizing staining protocols for laser microdissection of specific cell types from the testis including carcinoma in situ.
Sonne SB, Dalgaard MD, Nielsen JE, Hoei-Hansen CE, Rajpert-De Meyts E, Gjerdrum LM, Leffers H.

 Quantitative RT-PCR gene expression analysis of laser microdissected tissue samples.
Erickson HS, Albert PS, Gillespie JW, Rodriguez-Canales J, Marston Linehan W, Pinto PA, Chuaqui RF, Emmert-Buck MR.

Obtaining High Quality RNA from Single Cell Populations in Human Postmortem Brain Tissue.
Charmaine Y. Pietersen1, Maribel P. Lim1, Tsung-Ung W. Woo

A video article published in the Journal of Visualized Experiments which explains the staining and cutting methodology used.

Improved RNA preservation for immunolabeling and laser microdissection.
Brown AL, Smith DW.


Selected books on laser microdissection.

Laser capture microdissection: methods and protocols / edited by Graeme I. Murray and Stephanie Curran

This links to the electronic version of the book available from The Ohio State University Library. This resource is available only to OSU faculty, staff and students.







Other LCM Core Labs

There are several LCM Core labs that are an excellent resource for all researchers interested in laser microdissection. You can find novel methodologies, protocols and tips for enhancing your study.

East Carolina University Laser Capture Microdissection Core Laboratory

Laser Capture Microdissection facility at the Montana State University

The Special Techniques Group in the Cellular and Molecular Pathology Branch of the National Institute of Environmental Health Sciences (NIEHS) of the NIH.



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